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KMID : 0903519870300010077
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1987 Volume.30 No. 1 p.77 ~ p.87
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Studies on Production of Heteropolysaccharide by Mutant of Xanthomonas malvacearum
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Lee Ke-Ho
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Abstract
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The mutant with high productivity, X. malvacearum SNUF 560-6, was acquired from the X. malvacearum SNUF 560 with low productivity by UV-light irradiation. It was preserved is lyophilized stock culture and it was transferred to PD1 slant to maintain viability fortnightly. Fermentations were started by retransfering to MY agar slant from PDA stok culture. The experiments for optimal xanthan gum production were studied in a chemically defined medium. Of the carbon and nitrogen sources tested, 0.4% sucrose medium and 10mM glutamic acid medium yielded the highest xanthan gun production respectively. The addition of 10g/ℓ succinic acid stimulated xanthan gum production. Also 65mM PO₄^(-3) (12.6g/ℓ KH₂PO₄) was effective on xanthan gum production. Finally, medium 1 and medium 2 which have high xanthan gum production potencies were achieved in this stud.
The components of medium 1 and medium 2 were as follows:
Medium 1 : sucrose 40g/ℓ glutamate 10mM
PO₄^(-3) 54mM (KH₂PO₄ 12.65g/ℓ)
Citrate 2g/ℓ
WgSO₄·7H₂O 0.2g/ℓ
H₃BO₃ 0.005/ℓ
ZnO 0.006/ℓ
FeCl₂·6H₂O 0.0024g/ℓ
CaCO₃ 0.02g/ℓ
Medium 2 : Sucrose 40g/ℓ (NH₄)₂SO₄2g/ℓ
PO₄^(-3) 65mM (KH₂PO₄ 12.65g/ℓ)
Succinate 10g/ℓ
MgSO₄·7H₂O 0.02g/ℓ
H₃BO₃ 0.06g/ℓ
ZnO 0.006g/ℓ
FeCl₂·6H₂O 0.0024g/ℓ
CaCO₃ 0.02g/ℓ
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